inpouch tv culture system  (BioMed Diagnostics Inc)

Journal: Infectious disease clinics of North America

Article Title: Trichomoniasis

doi: 10.1016/j.idc.2023.02.001

Figure Lengend Snippet: 2A: 96-well plate used for 5-nitroimidazole drug susceptibility testing. 2B: Graphical representation of a portion of the 96 well plate for the 5-nitroimidazole drug susceptibility testing. Rows A and H are DMSO control lanes, while lanes B-G contain the 5-nitroimdazoles tested for specific T. vaginalis isolates tested in triplicates. 50 μl of media is initially added to each well. Next, 50 μl of drug/media is added to column 1 rows B-G, while 50 μl of DMSO/media are added to A and H and then serially diluted from left to right (column 1 [400 μg/ml] to column 12 [0.2 μg/ml]). 150 μl of the T. vaginalis sample is added to each well from the lowest concentration to the highest (column 12 [0.2 μg/ml] to column 1 [400 μg/ml]). The plates are then incubated for 48 to 52 hours before being read on an inverted microscope. MLC is determined when no motile TV is observed for all three wells with the same concentration.

Article Snippet: The InPouch ® T. vaginalis culture system (BioMed Diagnostics, White City, OR) is the most commonly used culture method and has a sensitivity of 44-81% and specificity of 100%.

Techniques: Concentration Assay, Incubation, Inverted Microscopy

Journal: Infectious disease clinics of North America

Article Title: Trichomoniasis

doi: 10.1016/j.idc.2023.02.001

Figure Lengend Snippet: Diagnostic tests for Trichomonas vaginalis

Article Snippet: The InPouch ® T. vaginalis culture system (BioMed Diagnostics, White City, OR) is the most commonly used culture method and has a sensitivity of 44-81% and specificity of 100%.

Techniques: Diagnostic Assay, Microscopy, Incubation, Solana Trichomonas Assay, AmpliVue Trichomonas assay